Zapoteca tetragona is a leguminous tree which grows fast and regrows well after several cuttings. Z tetragona is potential as a protein source because of its high protein content. It is, however, still underutilized as ruminant feed. Therefore, nutritional evaluation has to be conducted as the secondary compounds in the leaves may give beneficial or negative effect on ruminant production.

New Alternative Forage; Zapoteca Tetragona As Protein Source For Ruminant The evaluation consisted of chemical analysis, in vitro and in vivo experiments Secondary compounds including total phenol, condensed tannin and non protein amino acid (NPAA) were analysed. Two stage in vitro fermentation was conducted using substrates with increasing level of Ztetragona (15, 30,45 and 100 percent) replacing EG. Inclusion of 30 percent Z tetragona in EG was compared to 100 percent EG by in vitro fermentation and in vivo experiments using sheep. The in vivo experiment was conducted for 21 days and the last 7 days, offered and residual feed, feces, urine were collected and measured daily. DM and CP digestibility were calculated. The rumen liquor was collected at the end of experiment and was analyzed for its ammonia content, protozoa and total bacteria population. Proximate analysis of Z tetragona showed that the level CP was quite high (23.49 percent). Total phenol, condensed tannin and NPAA contents were very low ie 3.31 percent, 0.73 percent and 0.6 percent, respectively. In vitro gas test showed that after 48 hours incubation, the gas production of substitution 30 percent Z tetragona was higher than 100 percent EG (83.35 ml and 72.2 ml). Inclusion of increasing level of Z tetragona (15, 30, 45 and 100 percent) gave similar DM and CP digestibility values. Overall DM and CP digestibilities substitution Z tetragona were higher than those of 100 percent EG. In vivo experiment showed that there was no significant difference in DM intake between 100 percent EG (682.56 g/day) and 30 percent Z. tetragona (795.16 g/day), however there was higher protein intake when feeding 30 percent Z tetragona (122. 63 g/day) compared to 100 percent EG (60.54 g/day). DM digestibility among 2 treatments was not significantly different (30 percent Z tetragona : 62.23 percent and 100 percent EG : 56.58 percent) but CP digestibility increased significantly at 30 percent Z tetragona (71.45 percent) than 100 percent EG (56.53 percent). There was hardly any difference in rumen condition expect protozoa number increased with Ztetragona (1.04 X 105) compared to 2.07 X l04 in EG. It can be concluded that Z tetragona has a good nutritional value, and could be used as a protein source for ruminant.  Oleh: Bansi Hadriana; Wina E; Matitaputy Procula Rudlof. (The Assessment Technology of Agriculture Institute Maluku; Indonesian institute for Animal Production (Indonesian)). Proceeding The 4 th International Confrence on Sustainable Animal Agriculture For  Developing Conttres 27-31 Juli 2013. Lanzhou China. Year 2013,